Medical Definition of mischarge
: to attach an incorrect amino acid to during protein synthesis Benzer and coworkers showed in 1962 that cysteine tRNA mischarged with alanine (Ala-tRNACys) delivers alanine to growing polypeptide chains in vitro in response to cysteine codons.—David A. Tirrell, in Physical Biology: From Atoms to Science, 2008
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