Medical Definition of inoculate
1: to communicate a disease to (an organism) by inserting its causative agent into the body <12 mice inoculated with anthrax>
2a: to introduce microorganisms or viruses onto or into (an organism, substrate, or culture medium) <inoculated a rat with bacteria>b: to introduce (as a microorganism or antiserum) into an organism or onto a culture medium <inoculate a pure culture of bacteria into a healthy host>
3: to introduce immunologically active material (as an antibody or antigen) into especially in order to treat or prevent a disease <inoculate children against diphtheria>
: to introduce microorganisms, vaccines, or sera by inoculation
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