Medical Definition of gyrase
: a bacterial enzyme that is a topoisomerase which catalyzes the ATP-dependent breaking and rejoining of double-strands in circular DNA to generate negative supercoils that twist in the opposite direction as the turns in the original helices ◆The supercoils introduced by the bacterial gyrase help to relieve the torsional stress in the circular DNA strands that is a consequence of the unwinding of the DNA during replication.
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